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. 2018 Jan 24;52(3):815–827. doi: 10.3892/ijo.2018.4252

Figure 5.

Figure 5

Upregulation of miR-149-5p inactivates the Hippo signaling pathway in ovarian cancer cells. (A) Gene set enrichment analysis (GSEA) revealed that miR-149-5p expression positively correlated with the YAP and TAZ regulated signatures. (B) TEAD transcriptional activity was assessed by luciferase reporter constructs in the indicated cells. Error bars represent the means ± SD of 3 independent experiments; *p<0.05. (C) Western blot analysis of nuclear YAP and TAZ expression in the indicated cells. The nuclear protein p84 was used as the nuclear protein marker. (D) Real-time PCR analysis of CTGF, CYR61, HOXA1 and SOX9 in the indicated cells. Error bars represent the means ± SD of 3 independent experiments; *p<0.05. (E) Individual silencing of YAP and TAZ attenuated the mitochondrial potential of ovarian cancer cells increased by miR-149-5p overexpression. Error bars represent the means ± SD of 3 independent experiments; *p<0.05. (F) Individual silencing of YAP and TAZ increased the apoptotic rate of ovarian cancer cells which was decreased by miR-149-5p overexpression. Error bars represent the means ± SD of 3 independent experiments; *p<0.05. (G and H) Individual silencing of YAP and TAZ increased caspase-3 or caspase-9 activity in ovarian cancer cells which had been decreased by miR-149-5p overexpression. Error bars represent the means ± SD of 3 independent experiments; *p<0.05.