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. 2018 Jan 24;52(3):815–827. doi: 10.3892/ijo.2018.4252

Figure 6.

Figure 6

miR-149-5p targets MST1 and SAV1 in ovarian cancer cells. (A) Predicted miR-149-5p target sequence in 3′UTRs of MST1 and SAV1. (B) Western blot analysis of MST1 and SAV1 expression in the indicated cells. α-tubulin served as the loading control. (C and D) Luciferase assay of the cells transfected with pmirGLO-3UTR reporter in miR-149-5p overexpressing or silencing cells. Error bars represent the means ± SD of 3 independent experiments; *p<0.05. (E and F) miRNP IP assay showing the association between miR-149-5p and MST1 and SAV1 transcripts in the indicated cells. Pulldown of IgG antibody served as the negative control. Error bars represent the means ± SD of 3 independent experiments; *p<0.05. (G) Individual silencing of MST1 and SAV1 increased the mitochondrial potential of ovarian cancer cells which had been decreased by anti-miR-149-5p. Error bars represent the means ± SD of 3 independent experiments; *p<0.05. (H) Individual silencing of MST1 and SAV1 decreased the apoptotic rate of ovarian cancer cells which had been increased by anti-miR-149-5p. Error bars represent the means ± SD of 3 independent experiments; *p<0.05. (I and J) Individual silencing of MST1 and SAV1 decreased caspase-3 or caspase-9 activity in ovarian cancer cells which had been increased by anti-miR-149-5p. Error bars represent the means ± SD of 3 independent experiments; *p<0.05.