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. 2018 Jan 4;52(3):787–803. doi: 10.3892/ijo.2018.4236

Figure 7.

Figure 7

Transcriptional knockdown of MYCN proto-oncogene, bHLH transcription factor (MYCN) and fatty-acid binding protein 5 (FABP5) induces cellular apoptosis of IMR-32 cells. (A) The rate of late cellular apoptosis between MYCN, L1-cell adhesion molecule (L1-CAM), high mobility group protein A1 (HMGA1) and FABP5 siRNA-transfected and control IMR-32 cells did not differ significantly at 48 h post-transfection as determined using an Annexin V/propidium iodide (PI) cell apoptosis assay and flow cytometry analysis. (B) The rate of early apoptosis in MYCN and FABP5 siRNA-transfected IMR-32 cells was significantly higher compared to controls (~27 and ~29% compared to ~17%, respectively) at 48 h post-transfection. (C) Fluorescence microscopy images of MYCN and FABP5 siRNA-transfected cells stained with Annexin V (red) and PI (green) at 48 h post-transfection. Experiments were run in triplicates and repeated >3 times. Results represent the mean ± SEM.