Table II.

PCR primers.

Gene	Gene ID	Description	NCBI nucleotide reference	Primer sequences (forward and reverse)	Tm	PCR product length name (bp)
ABL1	25	ABL proto-oncogene 1	NM_005157.5	5′-TCATATCAACCCGAGTGTCT-3′ 5′-AACTTGTTCCTCATTTGCTG-3′	56°C 54°C	229
ANXA3	306	Annexin A3	NM_005139.2	5′-CGCAATCAGGTGGAGTCGAG-3′ 5′-TCACTAGGGCCACCATGAGA-3′	61°C 60°C	467
ACTB	60	Actin beta	NM_001101.4	5′-TCCCTGGAGAAGAGCTACGA-3′ 5′-AGGAAGGAAGGCTGGAAGAG-3′	59°C 59°C	98
CAT	847	Catalase	NM_001752.3	5′-CTCCGGAACAACAGCCTTCT-3′ 5′-GATGAGCGGGTTACACGGAT -3′	60°C 60°C	412
GAPDH	2597	Glyceraldehyde-3-phosphate dehydrogenase	NM_002046.5	5′-CCGTCTAGAAAAACCTGCC-3′ 5′-AGCCAAATTCGTTGTCATACC-3′	56°C 57°C	218
NPM1	4869	Nucleophosmin 1	NM_001037738.2	5′-GCGCATTGAACAGTCCTGGG -3′ 5′-CCAGCCTGAAGAGGCATGGGT -3′	62°C 64°C	172
PGK1	5230	Phosphoglycerate kinase 1	NM_000291.3	5′-GGGAAAAGATGCTTCTGGGAA-3′ 5′-TTGGAAAGTGAAGCTCGGAAA-3′	58°C 58°C	72
PPIA	5478	Peptidylprolyl isomerase A	NM_021130.4	5′-CTGGACCCAACACAAATGGT-3′ 5′-GCCTTCTTTCACTTTGCCAAAC-3′	58°C 59°C	98
S100A8	6279	S100 calcium binding protein A8	NM_001319196.1	5′-TGAAGAAATTGCTAGAGAC-3′ 5′-CTTTATCACCAGAATGAGGA-3′	50°C 52°C	131
S100A9	6280	S100 calcium binding protein A9	NM_002965.3	5′-CCTGGACACAAATGCAGACAA-3′ 5′-CGTCACCCTCGTGCATCTT-3′	59°C 60°C	101
STMN1	3925	Stathmin 1	NM_203401.1	5′-GCCCTCGGTCAAAAGAATCTG-3′ 5′-TGCTTCAAGACCTCAGCTTCA-3′	59°C 60°C	139
WT1	7490	Wilms tumor 1	NM_000378.4	5′-ACAGGGTACGAGAGCGATAACCA-3′ 5′-CACACGTCGCACATCCTGAAT-3′	63°C 61°C	105

The primer sequences of 3 reference genes (ACTB, PGK1 and PPIA) were from the study by Lossos et al (69). The STMN1 primers were from the study by Hussein et al (70). The WT1 primers were from the study by Kreuzer et al (71). The design of the remaining primers and melting temperature (T_m) calculation was supported by NCBI Primer Blast (www.ncbi.nlm.nih.gov/tools/primer-blast/).