| Bacterial and Virus Strains |
|
| Expression strain E. coli B834(DE3): F − ompT hsdSB (rB − mB −) gal dcm met (DE3) |
(Lossi et al., 2013) |
N/A |
|
| Chemicals, Peptides, and Recombinant Proteins |
|
| Vector for protein expression pET28-B1C1 |
(Lossi et al., 2013) |
N/A |
|
| Deposited Data |
|
| Cryo-EM map of P. aeruginosa TssB1C1 sheath |
This paper, deposited at EMDB |
EMD-3600 |
| Atomic model of P. aeruginosa TssB1C1 sheath |
This paper, deposited at PDB |
PDB 5N8N
|
|
| Software and Algorithms |
|
| MOTIONCORR |
(Li et al., 2013) |
http://cryoem.ucsf.edu/software/driftcorr.html |
| Gctf |
(Zhang, 2016) |
http://www.mrc-lmb.cam.ac.uk/kzhang/Gctf/ |
| RELION |
(He and Scheres, 2017) |
https://github.com/3dem/relion |
| ResMap |
(Kucukelbir et al., 2014) |
http://resmap.sourceforge.net/ |
| Coot |
(Emsley et al., 2010) |
https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/ |
| REFMAC |
(Murshudov et al., 1997) |
http://www.ccp4.ac.uk/ |
| PHENIX |
(Adams et al., 2010) |
https://www.phenix-online.org/ |
| MolProbity |
(Chen et al., 2010) |
http://molprobity.biochem.duke.edu/ |
| Rosetta |
(Leaver-Fay et al., 2011) |
https://www.rosettacommons.org/software |
| PyMOL |
Schrödinger, LLC |
https://pymol.org/ |
| UCSF Chimera |
(Pettersen et al., 2004) |
https://www.cgl.ucsf.edu/chimera/ |
| RaptorX |
(Wang et al., 2013) |
http://raptorx.uchicago.edu/ |
| PISA |
(Krissinel and Henrick, 2007) |
http://www.ebi.ac.uk/pdbe/pisa/ |
| MEGA |
(Kumar et al., 2016) |
http://www.megasoftware.net/ |
| RAMPAGE |
(Lovell et al., 2003) |
http://mordred.bioc.cam.ac.uk/∼rapper/rampage.php |
