L. plantarum Has to Be Alive to Express Its Full Potential to Sustain Drosophila Growth
(A and B) Larval longitudinal length at D7 AEL after dead/live bacteria inoculation on PYD. Live bacteria (1× living LpWJL or 100× living LpWJL) were inoculated once at D0 AEL. Heat-killed (HK) bacteria (1× HK LpWJL or 100× HK LpWJL) were inoculated once (single inoc., at D0 AEL) or three times (triple inoc., at D0, D3, and D5 AEL). (A) Asterisks represent statistically significant differences with GF larvae. (B) Asterisks above dot plots represent statistically significant differences with larvae inoculated once with 100× living LpWJL.
(C) Larval longitudinal length at D7 AEL of mex-GAL4 (mex>), UAS-labial-IR (>lab-IR) and mex-GAL4/UAS-lab-IR (mex>lab-IR) animals after bacterial association on PYD. Gray dot plots represent measurements of GF larvae; black dot plots represent measurements of mono-associated larvae. Asterisks represent statistically significant difference between GF and mono-associated larvae from the same genotype.
Asterisks illustrate statistical significance between conditions: ∗∗∗∗p < 0.0001, ∗p < 0.05, ns, not significant (p > 0.1).