Figure 1. The effect of hyperporination on growth of P. aeruginosa cells with different efflux capacities.

A. Growth curves of the indicated P. aeruginosa strains grown in the absence of the inducer. Overnight cultures were diluted 1:100 into a fresh LB medium; the cells were grown for 18 hours and OD₆₀₀ measured every 30 min. Data shown are the averages of three repeats and the error bars are SD (n=3). B. The same as in A but cultures were grown in the presence of 0.1 mM IPTG. C. After the growth curves were analyzed as described in A and B, cell aliquots were plated onto LB agar plates and CFUs counted after 24 hrs incubation at 37ºC. Averages of three repeats are shown with SD (n=3) as the error bars. D. Growth rates of indicated P. aeruginosa strains at increasing concentrations of IPTG. Averages of six repeats are shown with SD (n=3) as the error bars. E. Amounts of pyocyanin produced by indicated strains in the absence and presence of 0.1 mM IPTG. Test tubes containing overnight cultures of the indicated strains and their OD₆₀₀ readings.