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. Author manuscript; available in PMC: 2018 Feb 10.
Published in final edited form as: ACS Infect Dis. 2017 Nov 10;4(2):185–195. doi: 10.1021/acsinfecdis.7b00160

Figure 4. Expression MexG/HI-OpmD correlates with amounts of extracellular pyocyanin and is required for P. aeruginosa self-protection against its toxicity.

Figure 4

A. Extracellular concentrations of pyocyanin in PAO1, PΔ3, PΔ4, and PΔ6 parental strains (blue) and their derivatives lacking mexGHI-opmD (ΔGHID) mutants (orange). B. Extracellular concentrations of pyocyanin in cultures of PAO1 strains overexpressing MexEF-OprN or different combinations of MexG/HI-OpmD components. All measurements were done on cultures grown to stationary phase and in triplicate. Error bars are SD (n=3). C. Spot assays with pyocyanin showing zones of inhibition in strains with and without mexGHI-opmD. D. The same as C but with PMS. E and F. Quantification of zones of inhibition of pyocyanin (E) and PMS (F) in indicated strains. Error bars are SD (n=3).