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(A) Whole blood was obtained 5d and 1h before surgery, and 4h, 24h, and 72h after surgery. (B) Aliquots were stained with cell surface and intra-cellular antibodies and analyzed with mass cytometry. Plasma proteins were measured using a Luminex 63-plex assay. (C) Assays produced three data layers providing information about cell frequencies (red bar), cell signaling (green bar), and plasma protein concentrations (blue bar).
