Table 2.
Stability of candidate reference genes in response to biotic conditions.
| Biotic Conditions | Candidate Genes |
geNorm | Normfinder | BestKeeper | ΔCt | Recommendation | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Stability | Ranking | Stability | Ranking | Stability | Ranking | Stability | Ranking | |||
| Development stage | 18S | 0.674 | 1 | 0.596 | 1 | 0.54 | 1 | 1.2 | 1 | 18S, HSP70, 28S |
| 28S | 0.855 | 3 | 0.929 | 4 | 0.82 | 3 | 1.33 | 4 | ||
| ACTB | 1.21 | 6 | 1.09 | 6 | 1.03 | 7 | 1.46 | 6 | ||
| ATP1A1 | 1.174 | 5 | 0.997 | 5 | 0.93 | 4 | 1.4 | 5 | ||
| ATP6V1A | 1.078 | 4 | 0.861 | 3 | 1.09 | 8 | 1.33 | 3 | ||
| GAPDH | 1.34 | 8 | 1.201 | 8 | 0.93 | 5 | 1.55 | 8 | ||
| HSP70 | 0.674 | 1 | 0.75 | 2 | 0.76 | 2 | 1.25 | 2 | ||
| HSP90 | 1.268 | 7 | 1.157 | 7 | 0.99 | 6 | 1.5 | 7 | ||
| RP49 | 1.407 | 9 | 1.333 | 9 | 1.12 | 9 | 1.64 | 9 | ||
| Tissue | 18S | 0.138 | 1 | 0.455 | 5 | 0.14 | 1 | 0.73 | 3 | 28S, 18S, RP49 |
| 28S | 0.138 | 1 | 0.436 | 4 | 0.17 | 2 | 0.72 | 1 | ||
| ACTB | 0.73 | 8 | 0.939 | 8 | 0.98 | 8 | 1.09 | 8 | ||
| ATP1A1 | 0.579 | 6 | 0.381 | 3 | 0.45 | 5 | 0.77 | 5 | ||
| ATP6V1A | 0.871 | 9 | 1.272 | 9 | 1.2 | 9 | 1.36 | 9 | ||
| GAPDH | 0.526 | 5 | 0.568 | 6 | 0.66 | 7 | 0.82 | 6 | ||
| HSP70 | 0.451 | 4 | 0.373 | 2 | 0.42 | 4 | 0.74 | 4 | ||
| HSP90 | 0.637 | 7 | 0.592 | 7 | 0.55 | 6 | 0.87 | 7 | ||
| RP49 | 0.351 | 3 | 0.356 | 1 | 0.36 | 3 | 0.73 | 2 | ||
| Sex | 18S | 0.604 | 5 | 0.634 | 6 | 0.32 | 2 | 0.85 | 6 | HSP90, RP49 |
| 28S | 0.673 | 6 | 0.821 | 8 | 0.46 | 4 | 0.95 | 8 | ||
| ACTB | 0.746 | 8 | 0.601 | 5 | 0.69 | 8 | 0.82 | 5 | ||
| ATP1A1 | 0.821 | 9 | 0.999 | 9 | 1.02 | 9 | 1.08 | 9 | ||
| ATP6V1A | 0.368 | 3 | 0.424 | 4 | 0.58 | 6 | 0.72 | 4 | ||
| GAPDH | 0.706 | 7 | 0.768 | 7 | 0.35 | 3 | 0.93 | 7 | ||
| HSP70 | 0.197 | 1 | 0.372 | 3 | 0.6 | 7 | 0.7 | 3 | ||
| HSP90 | 0.453 | 4 | 0.231 | 1 | 0.18 | 1 | 0.67 | 2 | ||
| RP49 | 0.197 | 1 | 0.283 | 2 | 0.49 | 5 | 0.67 | 1 | ||