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. 2018 Feb 9;8:2768. doi: 10.1038/s41598-018-20915-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Co-localization analysis of ML1 and endosomal pathway specific antibodies: (A) Pearson’s coefficient frequency distribution of ML1 co-localization with caveolin or clathrin specific antibodies; (B) Pearson’s coefficient frequency distribution of ML1 co-localization with early, late endosome or lysosome specific antibodies; (C) Median Pearson’s coefficient of ML1 and endosomal pathway specific antibodies co-localization. CT26 cells were incubated with 10 µg/mL of ML1 for 2 h, then fixed, permeabilized and stained with specific antibodies.