Figure 6.

Analysis of antigen-specific T cells response. (A) Kinetics of nucleoprotein (NP)-specific T cell response in mice immunized with IDLV-NP/HA. IFNγ ELISPOT was performed using blood cells collected at 4, 12, and 24 weeks post-prime and splenocytes collected at 42 weeks post-prime. Results are expressed as mean spot forming cells (SFC) per 10⁶ cells. Cells were stimulated overnight with the MHC I-restricted epitope derived from NP protein sequence (black bars) or left untreated (white bars). Error bars indicate the SD among mice of the same group. (B) Analysis of HA-specific T cell response in immunized mice. IFNγ ELISPOT was performed using splenocytes collected at 42 weeks post-prime, using 10 pools of 15mers spanning the full length of HA protein, as described in Section “Materials and Methods.” Results are expressed as cumulative mean SFC per 10⁶ cells for each pool among mice of the same indicated group.