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. 2018 Feb 5;11:25. doi: 10.3389/fnmol.2018.00025

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Copyright © 2018 Filošević, Al-samarai and Andretić Waldowski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Locomotor sensitization to vCOC depends on time interval between exposures wile only limited number of individual flies in a population develops locomotor sensitization to vCOC. (A) Kinetic graph of average locomotion (counts per minute) for group of flies (n = 32) before administration (bsl), and after administrations of 75 μg vCOC first at 09:00 AM (1st) and then a second administration at 03:00 PM (2nd). Light gray panel 5 min before exposure, dotted line is time of the exposure, dark gray panel 5 min after exposure. (B) Histogram of different duration of time intervals between two administrations of vCOC (75 μg), plotted as a mean of population (32 flies) locomotor activity 5 min before (bsl) and 5 min after COC (1st) exposures at 09:00 AM and 2nd at different time points [12:00 AM (3 h), 03:00 PM (6 h), 05:00 PM (8 h), 09:00 PM (12 h), 09:00 AM of the following day (24 h) and 03:00 PM of the following day (30 h)], +SEM. The control (CTRL) group was exposed to warm air (8 min of heating, 1 min exposure, and 2.5 L/min air flow rate). Statistical significance (p ≤ 0.05) indicated by: c: within group comparison of bsl with 1st; d: 1st with 2nd; e: bsl with 2nd (t-tests for dependent samples). (C) Population response of the control group (CTRL) exposed to warm air and a group exposed twice to vCOC (COC 75 μg) using our standard protocol, with the first administration at 9:00 AM and the second at 3:00 PM. Data are plotted as mean of population (32 flies) locomotor activity 5 min before and 5 min after (1st and 2nd) exposure to COC, +SEM. Statistical significance (p ≤ 0.05) indicated by: c: comparison of bsl with 1st; d: 1st with 2nd; e: bsl with 2nd (within group comparison using t-test for dependent samples). (D) Analysis of individual flies from data in part (A). Percent of individual flies in a population that show sensitivity (SENS) and locomotor sensitization (LS) in control (CTRL) and group exposed to vCOC (COC 75 μg). ^∗χ² test statistical significance p ≤ 0.05. Starting data were same as (C) from which categories increase, decrease and same were divided.