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. 2018 Feb 5;9:68. doi: 10.3389/fphar.2018.00068

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Copyright © 2018 Han, Kee and Hong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rosmarinic acid (RA) exhibits an anti-proliferative effect on metastatic CRC cells. (A,B) The cell viability of RA-treated CT26 (A) and HCT116 (B) cells. The cells were seeded at a density of 3 × 10³ cells/well in 96-well microplates and treated with RA for 24, 48, 72, and 96 h. After treatment with RA, cell viability was measured by using the MTS assay. (C) The morphologies of CT26 and HCT116 cells treated with RA for 96 h and 72 h, respectively. The images were obtained by using a microscope (200× magnification). The results are expressed as the mean ± SD. ^∗p < 0.05 indicates a significant difference from the untreated group.