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. 2018 Feb 5;9:68. doi: 10.3389/fphar.2018.00068

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Copyright © 2018 Han, Kee and Hong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Rosmarinic acid (RA) regulates the EMT of CRC cells. (A,B) Representative immunofluorescence images of E-cadherin (A) and N-cadherin (B) in RA-treated CT26 cells for 48 h. The nuclei were stained with DAPI (blue) and the equivalent phase contrast images were taken by fluorescent microscope. (C,E) The mRNA expression of E-cadherin and N-cadherin in RA-treated CT26 cells (C) and HCT116 cells (E). (D,F) The mRNA expression of snail, twist, vimentin, and slug in RA-treated CT26 cells (D) and HCT116 cells (F). GAPDH and β-actin were used as the endogenous control. The results are expressed as the mean ± SD. ^∗p < 0.05 indicates a significant difference from the untreated group.