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. 2018 Feb 5;9:165. doi: 10.3389/fimmu.2018.00165

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Copyright © 2018 Hoppenbrouwers, Sultan, Abraham, Lemmens-den Toom, Hansenová Maňásková, van Cappellen, Houtsmuller, van Wamel, de Maat and van Neck.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Reversed effect of significantly reduced NETosis by Staphylococcus aureus knockout strain after the addition of purified Protein A. Except for S. aureus Newman ΔSpAΔSbi, all conditions induce more NETosis than Protein A only. Results of three separate experiments, neutrophils were derived from three individual donors. * indicates significant difference from S. aureus Newman ΔSpAΔSbi. ^# indicates significant difference when compared to neutrophils stimulated with 0.9 µg/ml Protein A (*/^#p < 0.05; **/^##p < 0.01; ***/^###p < 0.001).