Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Feb 9;9:594. doi: 10.1038/s41467-018-03010-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — RXEG1 mediates GH12 protein recognition in N. benthamiana. a Representative leaves showing cell death induced by expression of XEG1, INF1, NPP1, or Avh241 in N. benthamiana. Leaves (n = 10) were photographed three days after agro-infiltration (dai). Accumulation of each protein was monitored at 2 dai with anti-HA antibody. The numbers used for western blots match the numbers on the leaves. Protein loading is indicated by Ponceau S staining. Experiments were repeated three times with similar results. b Relative transcript levels of RXEG1 and RXEG-likes in TRV:GFP- and TRV:RXEG1-treated N. benthamiana. Leaves were collected from the same position of N. benthamiana of similar size and used for RNA isolation. The transcript levels were determined by qRT-PCR, normalized with EF-1α, and expressed as mean fold changes [±standard error (s.e.m.)] relative to TRV:GFP-treated leaves, which was set as 1. *, significant differences (P < 0.05, Student’s t-test). Similar results were obtained in three independent experiments. c Representative leaves showing expression of synthesized RXEG1 restored XEG1-induced cell death in N. benthamiana treated with TRV:RXEG1-1 or TRV:RXEG1-2. XEG1 was co-expressed with P19 and RXEG1^(S), RXEG1^LRR(S), RXEGL1/2^(S), or a mixture of RXEG1^(S) and RXEGL1/2^(S) in N. benthamiana leaves treated with TRV:GFP, TRV:RXEG1-1, or TRV:RXEG1-2. Infiltrated leaves (n = 6) was photographed at 3 dai. Total protein was isolated from infiltrated leaves at 2 dai and analyzed with anti-HA and anti-GFP antibodies. Protein loading is indicated by Ponceau S staining. Experiments were repeated three times with similar results. d Representative leaves showing cell death induced by expression of various GH12 proteins and INF1 in N. benthamiana leaves treated with TRV:GFP or TRV:RXEG1. GH12 proteins and INF1 were transiently expressed in N. benthamiana. Infiltrated leaves (n = 10) were photographed at 3 dai. Total protein was isolated from infiltrated leaves at 2 dai and accumulation of each protein was analyzed by western blot with anti-HA antibody. The numbers used for western blots match the numbers on the leaves. Protein loading is indicated by Ponceau S staining. Experiments were repeated three times with similar results