Fig. 3.

RXEG1 participates in XEG1-induced plant immunity. a, c Production of reactive oxygen species (ROS) in RXEG1-silencing (a) or overexpressing (c) N. benthamiana treated with 1 μm XEG1 or control (EV). Mean values (±s.e.m.) of four replicates are shown. Experiments were repeated twice with similar results. b Relative expression of defense-related marker gene CYP71D20 in RXEG1-silencing N. benthamiana leaves 3 h after treatment with 200 nm XEG1 or EV. Transcript levels were quantified by qRT-PCR and normalized to EF-1α. Bars represent the mean fold changes (±s.e.m.) of the XEG1-treated leaves relative to the value in EV-treated leaves, which was set as 1. *, significant differences (P < 0.05, Student’s t-test). Experiments were repeated three times with similar results. d, e Relative quantification of P. parasitica biomass using qPCR. Infected leaves (n = 12) were collected at 3 days post inoculation (dpi) and used for DNA isolation and qPCR analysis. *, significant differences (P < 0.05, Student’s t-test). Experiments were repeated twice with similar results