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. 2018 Feb 9;8:2710. doi: 10.1038/s41598-018-21182-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of higher doses of cocaine on GSH or NO level. Differentiated cells in phenol-red free medium were treated with PBS (control) or cocaine for 48 h. GSH (A) level in fixed cells (n = 7, **P = 0.0004) was measured as per established procedure at 412 nm in a micro plate reader; for NO detection (B), 100 μl of medium was transferred into a new 96-well plate (n = 9, P = 0.6) and mixed with equal volume of Griess reagent, followed by 10 min incubation in the dark. The absorbance readings at 540 nm were measured in a micro plate reader. Data are expressed as mean ± SEM and compared to the PBS controls.