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. 2018 Feb 9;8:2719. doi: 10.1038/s41598-018-21145-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MS profiling of site-specific glycoforms of the serum sFcγRIIIb, covering six N-glycosylation sites. (A) Asn38, (B) Asn45, (C) Asn64, (D) Asn74, (E) Asn162, and (F) Asn169. The spectra are based on the averaged mass scans of the glycopeptides containing individual N-glycosylation sites, which eluted in time ranges of (A) 27.8–29.1 min, (B) 32.7–33.4 min, (C) 31.0–33.8 min, (D) 21.8–24.8 min, (E) 32.0–39.0 min, and (F) 44.0–50.0 min. The identities of Man, Gal, Fuc, and GlcNAc were inferred from the literature and are represented by symbols according to the Symbol Nomenclature for Glycans (SNFG) system⁴⁰, the details of which can be found at NCBI (http://www.ncbi.nlm.nih.gov/books/NBK310273/): GlcNAc, ; Man, ; Gal, ; Neu5Ac, ; Fuc, .

Inline graphic — MS profiling of site-specific glycoforms of the serum sFcγRIIIb, covering six N-glycosylation sites. (A) Asn38, (B) Asn45, (C) Asn64, (D) Asn74, (E) Asn162, and (F) Asn169. The spectra are based on the averaged mass scans of the glycopeptides containing individual N-glycosylation sites, which eluted in time ranges of (A) 27.8–29.1 min, (B) 32.7–33.4 min, (C) 31.0–33.8 min, (D) 21.8–24.8 min, (E) 32.0–39.0 min, and (F) 44.0–50.0 min. The identities of Man, Gal, Fuc, and GlcNAc were inferred from the literature and are represented by symbols according to the Symbol Nomenclature for Glycans (SNFG) system⁴⁰, the details of which can be found at NCBI (http://www.ncbi.nlm.nih.gov/books/NBK310273/): GlcNAc, ; Man, ; Gal, ; Neu5Ac, ; Fuc, .