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. 2018 Feb 9;8:2691. doi: 10.1038/s41598-018-20928-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Toluidine blue stained image of the tcd1 coleoptile. The Raman-scanned areas are marked by red boxes. Visible images of (b) the epidermis and cortex; (c) vascular bundle; (d) emerging leaf; Corresponding Raman images (at 2800–3050 cm⁻¹) of the three tissue types in the tcd1 coleoptile (e–g). The 2245 cm⁻¹ nitrile (ν C≡N) band is absent in all tissues, indicating absence of dhurrin. (h) Average spectrum of 50 spectra randomly selected from the vascular bundle map. All material was prepared using cryosections.