Figure 5.

Transcriptome dynamics of UM-C162-treated and untreated S. aureus. (a) Hierarchically clustered expression profile (rows) for 456 S. aureus genes differentially expressed upon exposure to UM-C162 as compared to the untreated control. Data from three independent experiments (columns) are shown. (b) Volcano plot showing the gene level analysis to select differentially expressed genes after treatment with UM-C162. The data for all genes are plotted as fold change versus −log₁₀ of the adjusted p-value. Green, grey and red correspond to genes with < −2-fold, −2 to 2 fold and > 2-fold differential expression, respectively. (c) Verification of microarray data by qRT-PCR. Transcript levels of twelve differentially expressed S. aureus genes from qRT-PCR and microarray derived data were compared. Results displayed are the average relative fold-change for transcripts from UM-C162-treated bacteria compared to untreated control. The relative quantification of S. aureus RNA was determined by the change in expression of genes of interest normalized to S. aureus 16 s rRNA housekeeping gene.