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. 2018 Feb 9;8:2735. doi: 10.1038/s41598-018-20888-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical and qRT-PCR analysis of E18.5 embryonic liver following recombination at E14.5. (A) Schematic illustration of treatment regime showing time point of β-naphthoflavone injection (E14.5) and time point of tissue isolation for analysis (E18.5). (B) Haematoxylin & Eosin staining and immunohistochemical staining for β-catenin in embryonic liver sections taken from AhCre⁺Apc^+/+β-Cat^+l+, AhCre⁺Apc^+/flβ-Cat^+l+, AhCre⁺Apc^fl/flβ-Cat⁺¹⁺, AhCre⁺Apc^+/+β-Cat^+lfl and AhCre⁺Apc^+/+β-Cat^fllfl recombinants. (C) Immunofluorescent co-staining for CPSI (red) and GS (green) with DAPI counterstain (blue) in embryonic liver sections taken from the recombinants indicated. The magnification is x200, scale bars −50 µm. (D) qRT-PCR analysis of cDNA isolated from E18.5 embryonic liver following deletion of Apc and β-catenin at E14.5. Statistical changes and P values are indicated (***p < 0.0005; **p < 0.005).