Fig. 1.

DAO expression constructs. a Recombinant plasmids expressing GST-DAO fusion proteins used for immunization that were obtained by cloning different human DAO cDNA fragments into the bacterial expression vectors pGEX-5X-1/-2/-3, respectively. Grey bars indicate the positions of the copper amine oxidase N-terminal domains 2 and 3 (CuAO N2/N3), the enzymatic core domain (CuAO enzyme), and the signal peptide sequence (SP). b Recombinant plasmids expressing GST-DAO fusion proteins obtained by subcloning cDNA fragments from pGEX-huDAO02 into the bacterial expression vectors pGEX-5X-1/-2/-3, respectively. c 12.5% Silver-stained SDS polyacrylamide gel and immunoblot with an anti-GST antibody of lysates prepared from bacteria-harbouring plasmids pGEX-huDAO02-38 indicated on top of each lane. Migration positions of full-length fusion proteins are indicated by arrows, and their expected sizes are listed in Table 1. The sizes of molecular weight markers (M) are given on the left in kDa