Table 1.
Expression plasmids for huDAO fragments
| Plasmid | Vector | cDNA fragment | Peptide | FuP (kDa) |
|---|---|---|---|---|
| pGEX-huDAO01 | pGEX-5X-1 | SmaI66-SmaI566 | P23-R190 | 44.5 |
| pGEX-huDAO02 | pGEX-5X-3 | SmaI566-DraI1303 | R190-F435 | 53.1 |
| pGEX-huDAO03 | pGEX-5X-2 | DraI1303-EcoRI2255 | K436-V751 | 60.8 |
| pGEX-huDAO04 | pGEX-5X-3 | SmaI566-EcoRI2255 | R190-V751 | 87.8 |
| pGEX-huDAO11 | pGEX-5X-3 | SmaI566-Tsp45I1141 | R190-V381 | 47.1 |
| pGEX-huDAO12 | pGEX-5X-3 | SmaI566-ApaLI1015 | R190-V339 | 42.5 |
| pGEX-huDAO13 | pGEX-5X-3 | SmaI566-SacII861 | R190-R288 | 36.9 |
| pGEX-huDAO14 | pGEX-5X-3 | SmaI566-SacI652 | R190-E218 | 29.2 |
| pGEX-huDAO21 | pGEX-5X-1 | HaeIII583-HaeIII693 | Q196-W231 | 30.0 |
| pGEX-huDAO22 | pGEX-5X-3 | HaeIII693-HaeIII895 | V233-G299 | 33.4 |
| pGEX-huDAO23 | pGEX-5X-1 | HaeIII919-HaeIII1129 | P308-G377 | 33.7 |
| pGEX-huDAO24 | pGEX-5X-1 | HaeIII1129-DraI1303 | L378-F435 | 32.4 |
| pGEX-huDAO31 | pGEX-5X-3 | HaeIII693-AvaII783 | V233-V262 | 29.3 |
| pGEX-huDAO32 | pGEX-5X-2 | AvaII783-HaeIII895 | V262-G299 | 30.2 |
| pGEX-huDAO33 | pGEX-5X-2 | AvaII792-HaeIII895 | D265-G299 | 29.9 |
| pGEX-huDAO34 | pGEX-5X-3 | HaeIII693-NlaIV731 | V233-G244 | 27.3 |
| pGEX-huDAO35 | pGEX-5X-3 | NlaIV731-NlaIV792 | P246-E264 | 28.1 |
| pGEX-huDAO36 | pGEX-5X-3 | NlaIV731-NlaIV831 | P246-E278 | 29.6 |
| pGEX-huDAO37 | pGEX-5X-1 | NlaIV792-NlaIV831 | P266-E278 | 27.4 |
| pGEX-huDAO38 | pGEX-5X-1 | NlaIV831-HaeIII895 | P279-G299 | 28.3 |
Human DAO cDNA fragments obtained with different restriction endonucleases were cloned in frame into the expression vectors pGEX-5X-1/-2/-3 to produce different size GST-DAO fusion proteins (FuP). Superscripts indicate position of restriction site on cDNA sequence (relative to A1 of translational start codon) and amino acid position (relative to M1 of precursor protein), respectively