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. 2018 Feb 9;8:2717. doi: 10.1038/s41598-018-21252-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of central administration of CHA on the hypothalamic neurons. Representative photographs show cellular activation marker c-Fos (green), orexin (ORX) or melanin concentrating hormone (MCH) immunoreactivity (red), and merged in the hypothalamus of vehicle-injected WT mice (top row), 0.02 nmol of CHA-injected WT mice (second row), 0.02 nmol of CHA-injected ORX-AB mice (third row), and 0.2 nmol of CHA-injected ORX-AB mice (bottom row). Horizontal bar indicates 500 µm. Note absence of orexin-immunoreactivity in ORX-AB mice (left end column, bottom 2 rows).