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. 2018 Feb 9;8:2693. doi: 10.1038/s41598-018-21006-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Vinculin variants exhibit altered conformational plasticity. Vinculin, metavinculin, vinculin-T12 and vinculin-T12-A974K were subjected to IM-MS analysis and their collision cross section was calculated. (A) Representative MS dimension projections of the four vinculin variants emphasizing the variability in charge state distribution. Both vinculin-T12 and to a larger extent metaviculin, display a wide distribution of charge states (29⁺–55⁺) compared to vinculin and T12. This heterogeneous, highly charged population indicates the existence of extended conformers. (B) Experimental CCS values. The high charge states of metavinculin display a significantly larger CCS value compared to the lower charge states (17⁺–28⁺) or to the CCS values obtained for vinculin and vinculin-T12. Error bars represent the standard deviation of 3 different wave heights.