Table 1.

Hydrogen bond interactions at the Vt-D4 interface as seen in the crystal structures of vinculin, metavinculin and vinculin-T12-A974K (Å).

vinculin1		metavinculin		vinculin-T12-A974K
T973/O – E775/N	2.89 2.75	T1041/O – E775/N	3.26	T973/O – E775/N	3.65
D974/Oδ – N773/Nδ	3.04 3.04	D1042/Oδ – N773/Nδ	3.26
K975/N – N773/O	2.84 3.01	K1043/N – N773/O	3.03	A975/N – N773/O	3.28
K975/Nζ – E772/O	2.35 2.29	K1043/Nζ – E772/O	3.02
R976/N – N773/Oδ	2.94 3.03	R1044/N – N773/Oδ	3.01	A976/N – N773/Oδ	3.63
R978/Nh – E775/Oε	2.62/2.712 3.00/3.192	R1046/Nh – E775/Oε	2.47

¹For vinculin two values are listed for the independent polypeptide chains in the asymmetric unit of structure 1TR2.

²bifurcated hydrogen bond.