Table 1.
shRNA-based screen identifies nine positive autophagy regulators using restrictive analysis criteria
| Gene | # Scoring shRNAs | Avg Geomean |
|---|---|---|
| Atg7 | 5 | 21.60 |
| Atg16l1 | 4 | 7.59 |
| Birc6 | 4 | 3.18 |
| Rb1cc1 | 3 | 14.33 |
| Tsg101 | 3 | 7.15 |
| Atg12 | 3 | 4.91 |
| Sqstm1 | 3 | 3.77 |
| Nedd8 | 3 | 3.05 |
| Atg9a | 3 | 3.03 |
Hit list showing genes with highest scoring shRNAs, sorted based on the number of scoring shRNAs (# scoring shRNAs) and the average geometric mean (Avg Geomean) of the fold enrichment of shRNAs in the “GFP high” gate, compared with the “GFP low” gate. Established autophagy regulators are shown in bold