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. 2018 Feb 5;9:61. doi: 10.3389/fimmu.2018.00061

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Copyright © 2018 Xia, Kong, Zhou, Wu, Yao, He, He and Peng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the TgROP18-interacting proteins by SE-FRET assay. (A) Co-localization and FRET interaction of ROP18_I with NMI, IL20RB, P2RX1, IL21, and UBC. Localization and co-localization of ROP18_I and the five indicated candidates are shown in the donor channel (column 1) and the acceptor channel (column 2), respectively. The FRET efficiency is shown in column 3, in which a thermal pseudo color-matched FRET signal intensity scale is indicated for each image. (B) Quantitative analysis of FRET efficiency and intermolecular distance between ROP18_I and the five indicated candidates. Error bars represent the means ± SD of triplicates. Student’s t-tests results are between the six experimental groups and NC, *p < 0.05; **p < 0.01; ***p < 0.001. Abbreviations: NC, negative control; PC, positive control.