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. 2018 Feb 5;9:61. doi: 10.3389/fimmu.2018.00061

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Copyright © 2018 Xia, Kong, Zhou, Wu, Yao, He, He and Peng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of the TgROP18-interacting proteins by co-immunoprecipitation (Co-IP) assay. (A–D) Lysates of COS-7 cells co-overexpressing ROP18_I and the indicated interacting proteins were immunoprecipitated with the indicated antibodies. Rabbit, mouse, or goat normal control IgG were used as negative controls. The immunoprecipitates were detected by SDS-PAGE and western blotting using the antibodies indicated. (E) Lysates of COS-7 cells overexpressing ROP18_I in the presence of MG132 (10 μM) for 12 h were immunoprecipitated with the anti-FLAG antibody. Endogenous UBC (a smear of bands) was detected in the immunoprecipitates through western blotting with anti-FK2 antibody, which recognizes mono- and polyubiquitinylated conjugates.