Figure 5.

IFNAR signaling affects the Leishmania major-induced production of interferon (IFN)-α/β and interleukin (IL)-12 in BM-plasmacytoid dendritic cells (pDCs), but not bone marrow-derived dendritic cells (BM-mDCs). CD11b⁺CD11c⁺CD86⁻ sorted BM-mDCs and B220⁺CD11b^intCD11c⁺ sorted BM-pDCs were cultured for 48 h with L. major promastigotes (multiplicity of infection = 3), 200 ng/ml LPS, 1 µM CpG-oligodesoxynucleotides (ODN) 2216 or CpG-ODN 1668. Culture supernatants were analyzed for IFN-α/β (vesicular stomatitis virus bioassay with L929 fibroblasts) and IL-12p40/p70 content (ELISA). Triangles depict values below the detection limit of the assays. (A) BM-mDCs, (B) BM-pDCs. Mean results (±SEM) of three to four independent experiments are shown. Asterisks depict significant differences between wild-type (WT) and IFNAR1^−/− cells (*p < 0.05; Mann–Whitney test).