Fig. 4.

Global DNA methylation and hydroxymethylation analysis after exposure to MWCNTs and SWCNTs. After exposure to MWCNTs and SWCNTs at the selected doses, the whole genomic DNA within the cells were measured by means of (a) DNA methylation (5-mC) and (b) hydroxymethylation (5-hmC) using LC-MS/MS technique. No significantly different effects were detected between the various conditions. 25 and 100 represents exposure concentrations as μg/ml. Statistics were performed using one-way ANOVA, Dunnett’s multiple comparison test. Only decitabine-exposed cells decreased DNA 5-mC % in comparison to vehicle-exposed cells. ** indicates p value < 0.01