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. 2018 Jan 4;9(2):78–83. doi: 10.1021/acsmedchemlett.7b00363

Figure 5.

Figure 5

Suppression of lipid accumulation by 10 in adipocytes. (A) Cell toxicity of 10 on 3T3-L1 adipose cells. The cells were incubated for 6 days in DMEM with various concentrations of 10 (0–10 μM), and cell toxicity was measured. Data represent means ± SD (n = 3 independent experiments). (B) Staining of intracellular lipids by Oil Red O in 3T3-L1 cells. Cells (undifferentiated cells: U) were differentiated into adipocytes [D] for 6 days in DMEM with 10 (0–5 μM) or guggulsterone (Gu: 10 μM) together without (upper) or with CDCA (5 μM; lower). Intracellular lipid droplets were stained with Oil Red O. Data are representative of n = 3 repetitions. (C) Decrease of the intracellular triglyceride level by 10 in 3T3-L1 cells. The cells (undifferentiated cells: U; white column) were differentiated [D] into adipocytes for 6 days in DMEM without (gray column) or with 10 (0.5, 1, or 5 μM; black columns), or guggulsterone (Gu: 10 μM; purple columns) together without or with CDCA (5 μM; blue column). Data are presented as means ± SD from n = 3 experiments. *p < 0.01 (vs undifferentiated cells), #p < 0.01 (vs vehicle-treated differentiated cells), and +p < 0.01 (vs CDCA-treated differentiated cells).