Figure 7.

Comparison of expression determined by RNA-Seq and qPCR on 12 genes in soybean roots and nodule development. Half of the roots and nodules were inoculated with B. japonicum strain 113-2 and harvested at different time intervals post inoculation, and the other roots treated with water served as the uninoculation control. The relative expression levels of roots were calculated with reference to the value of the control roots harvested 0.5 h after mock treatment with water, and the relative expression levels of nodules were calculated with reference to the value of the branching_stage N. All qPCR reactions were repeated three times and the data are presented as the mean ± SD.