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. 2018 Feb 6;12:23. doi: 10.3389/fncel.2018.00023

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Copyright © 2018 Michalski, Keck, Grosche, Martens and Härtig.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative ischemia-related alterations of oligodendrocyte structures and the vasculature in the striatum 1 day after experimental focal cerebral ischemia in 3-month-old (C) as well as in 12-month old mice (A,B), captured by laser scanning microscopy. Concomitant fluorescence staining of the oligodendrocyte-specific protein (OSP, A) and the myelin basic protein (MBP, A′) as myelin-associated markers exhibited a dense network of dotted and strand-like structures, which appeared visually enhanced due to ischemia. Immunoreactivity of the neuron-glia antigen-2 (NG2) was nearly absent in these tissues (B), while simultaneous detection with MBP clearly showed enhancement of the MBP in the same region (B′). Endogenous serum albumin was found in ischemia-affected areas (C) indicating impaired blood – brain barrier integrity, while visualization of the vasculature by collagen IV (Coll IV)-immunostaining (C′) and detection of Solanum tuberosum lectin (STL)-binding sites (C″) appeared robustly with a wide range of overlapping vascular structures (C″′). Scale bars: A′ (also valid for A) = 50 μm, B′ (also valid for B) = 50 μm, C″′ (also valid for C–C″) = 25 μm.