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. 2018 Feb 6;12:23. doi: 10.3389/fncel.2018.00023

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Copyright © 2018 Michalski, Keck, Grosche, Martens and Härtig.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Representative forebrain overview scans from immunolabeled myelin basic protein (MBP) 1 day after focal cerebral ischemia in 3-month-old (A) and a 12-month-old mice (B). The enhanced MBP-immunosignal captured the overall territory of the middle cerebral artery (i.e., the ischemia-affected striatum, some thalamic regions and lateral parts of the neocortex), which was occluded by a filament-based model of focal cerebral ischemia. Higher magnification (inset in B) indicated dense packages of MBP-immunopositive structures (arrows with plain lines) predominantly in subcortical regions and – toward cortical areas – groups of MBP-positive, mostly parallel arranged strands (arrows with dashed lines). Scale bars: (A,B) = 1 mm.