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. 2016 Nov;18(6):903–911. doi: 10.1016/j.jmoldx.2016.07.004

Figure 2.

Figure 2

EGFR T790M assessment by dPCR. A total of 71 cases were tested. Initial assessment was based on a high-sensitivity LNA-PCR Sanger Sequencing assay to establish the T790M status. Cases were divided into two sets. Set 1 encompassed cases submitted for comprehensive testing by NGS. Set 2 encompassed cases in which MSK-IMPACT testing was not ordered. dPCR was performed on both sets either based on MSK-IMPACT libraries or directly from DNA. For a subset of cases in set 1 (28 cases) testing was also performed on direct DNA based on availability for paired direct comparisons. Among cases tested on the libraries, there was 100% success. For cases tested directly from DNA, one case was nonevaluable and two (from the paired set 1) were borderline. dPCR, digital PCR; LNA, locked nucleic acid; MSK-IMPACT, Memorial Sloan-Kettering-Integrated Mutation Profiling of Actionable Cancer Targets; NGS, next-generation sequencing.