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. 2018 Feb 6;11:18. doi: 10.3389/fnmol.2018.00018

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Copyright © 2018 Everington, Gibbard, Swinny and Seifi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Early life stress (ELS)-induced changes in the level of GABA_AR subunit mRNA expression within various peripheral organs. Quantification of the ELS-induced changes in the expression levels of the (A) α1-GABA_AR subunit mRNA, (B) α2-GABA_AR subunit mRNA, (C) α3-GABA_AR subunit mRNA, (D) α4-GABA_AR subunit mRNA, (E) α5-GABA_AR subunit mRNA, and (F) γ2-GABA_AR subunit mRNA, within mouse stomach (St), lung (Lu), bladder (Bl), kidney (Ki), heart (Hr), and liver (Li) tissue, relative to the housekeeping gene GAPDH, using qPCR. Note that the mRNA expression of some subunits, relative to GAPDH, was not always detectable (ND) using qPCR. Data represent mean ± SEM; N = 5 animals. NS, not significant. Student’s unpaired t-test. ^∗P < 0.05.