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. 2018 Feb 8;6:e4355. doi: 10.7717/peerj.4355

Table 1. Summary of amplicon, restriction enzyme, restriction site, and respective fragment sizes (bp, base pairs) differentiating Hawaiian Pocillopora species.

Species Amplicon Restriction enzyme site Fragment sizes (bp) Amplicon Restriction enzyme site Fragment sizes (bp) Cuts other species? Y/N (Fragment sizes bp)
P. eydouxi (1) mtORF SacI GAGCT’C C’TCGAG 298, 680 (2) PocHistone XhoI C’TCGAG GAGCT’C 287, 382 N
P. meandrina (1) mtORF SacI GAGCT’C C’TCGAG 298, 680 (2) PocHistone XhoI C’TCGAG GAGCT’C 669 N
P. verrucosa (1) mtORF AciI C’CGC GGC’G 209, 338, 431 Y, all species (430, 548)
P. ligulata (1) mtORF AlwNI CAGNNN’CTG GTC’NNNCAG 467, 511 Y, mtORF 11 (467, 511)
P. acuta (1) mtORF NlaIV GGN’NCC CCN’NGG 30, 171, 315, 462 (2) mtORF Tsp45I ‘GTSAC CASTG’ 978 Y, NlaIV: mtORF 1, 2, 6, 8 (201, 315, 462) Y, NlaIV: mtORF 3i (194, 784) Y, NlaIV: mtORF 3 (516, 462)
P. damicornis (1) mtORF NlaIV GGN’NCC CCN’NGG 30, 171, 315, 462 (2) mtORF Tsp45I ‘GTSAC CASTG’ 530, 448 Y, NlaIV: mtORF 1, 2, 6, 8 (201, 315, 462) Y, NlaIV: mtORF 3i (194, 784) Y, NlaIV: mtORF 3 (516, 462) Y, Tsp451: mtORF 3f (204, 774)