Table 2.

DNA Sequences

Primer/fragment	Sequence
Not_FraxC	5′-AGTTCAGCGGCCGCGCTCAGCTCCGTTTCGGTTTCACTTCCGGT-3′
Not_FraxR4	5′-CAAGTCGCGGCCGCCTTGTAGAAAGCGCCATTGGAGCCCCGCA-3′
Fmr1_CCG5	5′-AGCGTCTACTGTCTCGGCACTTGCCCGCCGCCGCCGCCG-3′
Fmr1_CGG5	5′-AGCGTCTACTGTCTCGGCACTTGCCGGCGGCGGCGGCGG-3′
FMR1 exon 1 (forward)	5′-GAACAGCGTTGATCACGTGAC-3′
FMR1 exon 1 (reverse)	5′-GTGAAACCGAAACGGAGCTGA-3′
GAPDH exon1 (forward)	5′-TCGACAGTCAGCCGCATCT-3′
GAPDH intron1 (reverse)	5′-CTAGCCTCCCGGGTTTCTCT-3′
Internal methylation control fragment	1 GCTCAGCTCC GTTTCGGTTT CACTTCCGGT AGCACGTCGC GGGGCGCCGT 51 AACGGCGAAT CGAGGGCGCG CACCGCCGTG ACATCGGCGT CATTCGGTCG 101 CATCACGGTT GTCGCCGGTG CGCGGTCGGA AAAGCGGGCG CGAGGCAGCG 151 GCGACGGCGC GAGTGAATCT AACGGGCGAC AACGCGTTCG GCCTCGGAGT 201 GGCGGCCACG GGTATGCGGG GCTCCAATGG CGCTTTCTAC AAG

Sequences in bold are not homologous to the FMR1 gene. The primer sequences are underlined, whereas the 2 HpaII sites within the amplicon are italicized.