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. 2016 Apr;186(4):938–951. doi: 10.1016/j.ajpath.2015.11.027

Figure 6.

Figure 6

IFN-λ expression by transfection activates the induced pStat1 and ISG expression in HCV-infected Huh-7.5 cells. HCV-infected Huh-7.5 cells (C/T) at day 6 were transfected with mammalian expression plasmid that contained full-length cDNA clone for IFN-λ1 (IL29), IFN-λ3 (IL28B), or IFN-λ4 or pcDNA3.1 vector alone at various concentrations. After 24 hours, protein extracts were prepared and examined for the expression of IFN receptor, Stat phosphorylation, and ISG expression by Western blot analysis. The induction of IL28B, IL29 in IFN-λ4–transfected culture was examined by Western blot analysis. GAPDH levels were monitored as a loading control. HCV, hepatitis C virus; IFN, interferon; ISG, IFN-stimulated gene.