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Gating strategy for germinal center B cells and plasma cells. Live single-cell splenocytes were gated on B220+ cells that were further gated on GL7+FAS+ cells to identify and enumerate germinal center B cells. B220+CD138+ cells were defined as plasmablasts, whereas B220−CD138+ cells were defined as plasma cells. Top panels: Cells from 564+/+ mice (8 months). Bottom panels: B6 controls (4 months). Numbers indicate percentages of total cells.
