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Gating strategy for Tfh cells. Live single-cell splenocytes were gated on CD4+ cells and thereafter on ICOS+ cells. CD4+ ICOS+ cells were further gated on PD-1+CXCR5+ cells to identify and enumerate Tfh cells. Numbers in the contour plots indicate percentage of total cells. VAMN, vancomycin, ampicillin, metronidazole, and neomycin.
