Figure 5.

Transcriptome analysis of spleens from 564^+/+ mice. Total RNAs from 4-, 8-, and 12-month-old 564^+/+ mice and B6 mice were examined for gene expression profiles with biological replications of three to four mice per group. A: Plot from principle component analysis is shown. Each ball represents an individual mouse. B: Source of variations in one-way analysis of variance. Differentially expressed genes were categorized based on their biological functions, and the enrichments were tested for statistical significance by Fisher's exact test. C: The top five significant categories. Members in the humoral immune response shown (C) are displayed in the network, and activities of detailed biological processes were predicted for activation or inhibition using Ingenuity Pathway Analysis algorithms (D).