Table 1.
List of Antibodies Used in Immunohistochemistry, FACS, and ELISA
| Antibody | Application | Clone | Format | Source (location) |
|---|---|---|---|---|
| B220 | FACS | RA3-6B2 | APCCy7, Alexa 700 | Biolegend (San Diego, CA) |
| IgM | FACS | RMM-1 | PECy7 | Biolegend |
| CD19 | FACS | 6D5 | APCCy7, Alexa 700, Pacific Blue, APC | Biolegend |
| CD5 | FACS | 53-7.3 | PECy5 | Biolegend |
| CD23 | FACS | B3B4 | PE | BD Biosciences (San Jose, CA) |
| CD21 | FACS | 7E9 | FITC, APCCy7 | BD Biosciences |
| PNA | FACS | NA | Biotin | Sigma (St. Louis, MO) |
| GL7 | FACS | GL7 | FITC | BD Biosciences |
| CD4 | FACS | GK1.5 | APCCy7, Alexa 700, Pacific Blue, APC, FITC | Biolegend |
| ICOS | FACS | C398.4A | PECy5 | BD Biosciences |
| PD-1 | FACS | 29F.1A12 | APC | BD Biosciences |
| CXCR5 | FACS | L138D7 | PE, Pacific Blue, PercpCy5.5 | BD Biosciences |
| IgM | ELISA | II/41 | Purified | BD Biosciences |
| IgM | ELISA | R6-60.2 | Biotin | BD Biosciences |
| IgG2b | ELISA | R9-91 | Purified | BD Biosciences |
| IgG2b | ELISA | R12-3 | Biotin | BD Biosciences |
| IgG1 | ELISA | A85-1 | Purified | BD Biosciences |
| IgG1 | ELISA | B68-2 | Biotin | BD Biosciences |
| B220 | IHC | RA3-6B2 | Purified | BD Biosciences |
| CD3 | IHC | CD3-12 | Purified | BioRad (Raleigh, NC) |
ELISA, enzyme-linked immunosorbent assay; FACS, fluorescence-activated cell sorting; FITC, fluorescein isothiocyanate; ICOS, inducible T cell co-stimulator; IHC, immunohistochemistry; NA, not applicable; PNA, peanut agglutinin.