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. 2017 Nov 14;118(3):416–420. doi: 10.1038/bjc.2017.404

Figure 3.

Figure 3

The effects of wild-type (WT) vs mutant (MT) SgI peptides on the growth of prostate cancer cells. LNCaP and C4-2 cells were transfected with Xfect control or a SgI peptide (250 ng) and cultured in medium supplemented with 10% normal FBS in the presence (A) or absence (B) of 100 μM (LNCaP)/300 μM (C4-2) zinc for 72 h. Cell viability assayed with MTT is presented relative to cell number in each control line (first lanes; set as 100%). Each value represents the mean+s.d. of three independent determinations. #P<0.05 (vs control cells). (C) Total proteins extracted from LNCaP or C4-2 cells transfected with Xfect control or a SgI peptide (2 μg) and cultured in medium supplemented with 10% normal FBS in the presence of 300 μM zinc for 24 h were analysed on western blots, using an antibody to PSA (33 kDa) or GAPDH (37 kDa).