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. 2017 Nov 16;118(3):428–434. doi: 10.1038/bjc.2017.409

Figure 5.

Figure 5

(A) SDS–PAGE (10% Coomassie Brilliant Blue staining) analysis of the effects of Td-CTLP on native soluble type I and II collagen degradation by MMP-8. Lane 1: 1.5 μM type I collagen; lane 2: type I collagen incubated with pro MMP-8; lane 3: type I collagen incubated with Td-CTLP-treated MMP-8; lane 4: type I collagen incubated with Td-CTLP; lane 5: type II collagen; lane 6: type II collagen incubated with MMP-8; lane 7: type II collagen incubated with Td-CTLP. α1, α2, and β1 indicate intact soluble native type I and II collagen monomers and dimers and βA and αA indicate their characteristic ¾-cleavage products resulting from collagenase activity. (B) Type I gelatin-zymographic analysis of the effects of Td-CTLP on human 92 kDa proMMP-9. Gelatinolytic 80–95 kDa Td-CTLP (lane 1) converts time-dependently (0–60 min) 92 kDa pro MMP-9 (lane 2) to 82 kDa gelatinolytically active form of MMP-9 (lanes 3–5, indicated by an arrow).