Fig. 4.

Effect of eASN on gene expression of selected Aβ secretases and metalloproteinases. The mRNA level of Adam10, Bace1, Psen1, Psen2 (a) and Mmp2,9,10,11 (b) after 24 h of 0,5 μM eASN treatment was measured with real-time PCR. The value expresses the fold of the above gene stimulation normalized against Actb (β-actin). Data represent the mean value ± S.E.M of four-six separate experiments with four replications. The relative level of mRNA was calculated by ΔΔCt method. **p<0.01 and ***p<0.001 versus control(phosphate buffer -treated PC12 cells) by Student’s t-test