Figure 5.

ER stress does not impair RA-driven PML-RARα degradation. (a) Western blot analysis of total extracts from NB4 cells with 10 nM RA with or without 50 ng/ml Tm, decorated with an anti-PML antibody. The empty arrowhead points to PML-RARα, the other bands are different PML isoforms. Actin was used as loading control. (b) Confocal microscopy analysis of NB4 cells treated for 72 h as in (a) stained with anti-Calnexin (CNX, green) and anti-PML PGM3 (red) antibodies. The white arrows point to differentiating cells with a reduced amount of microspeckles and an irregularly shaped nucleus indicating differentiation. (c) Western blotting in non-reducing conditions of total protein extracts NB4 cells treated as in a for 72 h decorated with an anti-RARα and, after stripping, with an anti-PML antibody. GAPDH was used as loading control.